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来源:Advances in Materials Science and Engineering , 2017

作者:Hai-le Ma;Lin Yang;Yu Shi;等

使用许可:署名(BY)

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The aim of this study was to develop a thermally and operationally stable trypsin through covalent immobilization onto chitosan magnetic nanoparticles (Fe3O4 @CTS). The successful preparation of the Fe3O4 @CTS nanoparticles was verified by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and transmission electron microscopy (TEM), which indicated that the prepared Fe3O4 @CTS nanoparticles have superparamagnetic properties, with an average size of approximately 17 nm. Then, trypsin was covalently immobilized onto the Fe3O4 @CTS nanoparticles at a high loading capacity (149.25 mg/g). The FTIR data demonstrated that the trypsin had undergone a conformational change compared with free trypsin, and the Michaelis constant (Km) and the maximum hydrolysis reaction rate (Vmax) showed that the trypsin immobilized on the Fe3O4 @CTS had a lower affinity for BAEE and lower activity compared with free trypsin. However, the immobilized trypsin showed higher activity than free trypsin at pH 6.0 and in alkaline conditions and retained more than 84% of its initial activity at 60°C after 8 h incubation. Its excellent performance across a broader pH range and high thermal stability, as well as its effective hydrolysis of bovine serum albumin (BSA) and its reusability, make it more attractive than free trypsin for application in protein digestion.

    来源:Advances in Materials Science and Engineering , 2017

    作者:Haifu Wang, Guoping Cen, Wantong Cai

    使用许可:署名(BY)

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    Fractal theory is introduced in fracture surface research of alkali-slag concrete (ASC) under freeze-thaw cycles; crack distribution of ASC fracture surface and freeze-thaw damage zone were calculated. Through fractal analysis of ASC sample fracture surfaces, relevance between section fractal dimension and fracture toughness and relationship between material composition and section fractal dimension are clarified. Results show that the specimen’s cracks before freeze-thaw extend along force direction gently, and there are more twists and turns after freezing and thawing; the fractal dimension D also grows from 1.10 to 1.33. SEM internal microcracks’ D of ASC internal microstructure after freezing and thawing is 1.37; 0 to 300 times ASC fractal dimension under freezing and thawing is between 2.10 and 2.23; with freeze-thaw times increasing, ASC fracture toughness decreases and fractal dimension increases, the fractal dimension and fracture toughness have a good linear relationship, and the fractal dimension can reflect the toughening effect of ASC. It is very feasible to evaluate ASC fracture behaviour under freezing and thawing with the fractal theory. Fractal dimension generally increases with activator solution-slag (A/S for short) or slag content. The greater the amount of A/S or slag content, the lower the dimension.

      来源:Advances in Materials Science and Engineering , 2017

      作者:Jung J. Kim, Mahmoud M. Reda Taha, Jiho Moon

      使用许可:署名(BY)

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      A polymeric hybrid composite system made of UHPC and CFRP was proposed as a retrofit system to enhance flexural strength and ductility of RC slabs. While the effectiveness of the proposed system was confirmed previously through testing three full-scale one-way slabs having two continuous spans, the slabs retrofitted with the hybrid system failed in shear. This sudden shear failure would stem from the excessive enhancement of the flexural strength over the shear strength. In this study, shear connectors were installed between the hybrid system and a RC slab. Using simple beam, only positive moment section was examined. Two full-scale RC slabs were cast and tested to failure: the first as a control and the second using this new strengthening technique. The proposed strengthening system increased the ultimate load carrying capacity of the slab by 70%, the stiffness by 60%, and toughness by 128%. The efficiency of shear connectors on ductile behavior of the retrofitted slab was also confirmed. After the UHPC top is separated from the slab, the shear connector transfer shear load and the slab system were in force equilibrium by compression in UHPC and tension in CFRP.

        来源:Advances in Materials Science and Engineering , 2017

        作者:Ahmad Asali;Thomas Schrefl;Johannes Bernardi;等

        使用许可:署名(BY)

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        来源:Advances in Materials Science and Engineering , 2017

        作者:Yanhu Mu;Shuping Zhao;Wei Ma;等

        使用许可:署名(BY)

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        The damage variable is very important to study damage evolution of material. Taking frozen loess as an example, a series of triaxial compression and triaxial loading-unloading tests are performed under five strain rates of 5.0 × 10−6–1.3 × 10−2/s at a temperature of −6°C. A damage criterion of frozen loess is defined and a damage factor Dc is introduced to satisfy the requirements of the engineering application. The damage variable of frozen loess is investigated using the following four methods: the stiffness degradation method, the deformation increase method, the dissipated energy increase method, and the constitutive model deducing method during deformation process. In addition, the advantages and disadvantages of the four methods are discussed when they are used for frozen loess material. According to the discussion, the plastic strain may be the most appropriate variable to characterize the damage evolution of frozen loess during the deformation process based on the material properties and the nature of the material service.

          来源:Advances in Materials Science and Engineering , 2017

          作者:Ching-Chuan Chang

          使用许可:署名(BY)

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          The purpose of this research is to investigate the behavior of a long bubbles penetrating through viscoelastic fluids in a curved tube. The injection gas flow is controlled by a mass flow controller (MFC). The results of the experiments show that the bubbles width approaches constant value at the location six-diameter upstream from the bubbles front. A difference variable is introduced at the six-diameter location to show the shifting deviation of the bubbles in the curved tube. It is shown that, with the same fluid viscosity and the curved angle, the difference is higher when the gas flow rate is higher. Also, the difference increases proportionally when the capillary number and the Weissenberg number increase.